Tissue Lysates
Genscript Corporation Alkaline Phosphatase Placenta
Alkaline phosphatase can be considered our favorite enzyme for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology Few might know however that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease
Sino Biological Recombinant Human Angiopoietin-2/ANGPT2 Protein (mFc Tag)
A DNA sequence encoding the Human ANGPT2 (NP_001138.1) (Tyr19-Phe496) was expressed with the Fc region of mouse IgG1 at the N-terminus.
Sino Biological Recombinant Canine FCGRT & B2M Heterodimer Protein (His & AVI Tag), Avi-tag Biotinylated, HPLC-verified
A DNA sequence encoding the Canine FCGRT (XP_038512242.1) (Ala23-Pro298) was expressed with a c-terminal AVI tagged polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding Canine B2M (NP_001271408.1) (Met1-Asn125) was fused , constructed the plasmid 2. The two plasmids were co-expressed and the Canine FCGRT & B2M Heterodimer was purified. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Sino Biological Recombinant Human IL-31 Protein (His & AVI Tag), MALS-verified
A DNA sequence encoding the Human IL31 (NP_001014358.1) (Ser24-Thr164) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
Sino Biological Norovirus GII.4 VP1 Protein (His & MBP Tag)
A DNA sequence encoding the Norovirus GII.4 (Norovirus Hu/GII.4/Sydney/NSW0514/2012/AU) VP1 (4OOS_A) (Thr1-Met309) was expressed with a N-terminal polyhistidine tag followed by a MBP tag.
Sino Biological Recombinant Human CD9 Protein (Nanodisc, Full-length, His-GFP Tag), Fluorescent
A DNA sequence encoding the Human CD9 (P21926) (Met1-Val228) was expressed with a N-terminal polyhistidine tag followed by a GFP Tag. Nanodisc is a versatile tool for studying membrane proteins. Using styrene-maleic acid (SMA) copolymer, membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better. Compared to membrane scaffold proteins (MSPs) nanodiscs, SMA nanodiscs also have the advantage of preserving proteins' nature by maintaining native lipids surrounded without introducing any heterologous proteins, which allows studies of protein structure and functions in a native-like environment.